Publications

*Heterologous flavivirus exposure provides varying degrees of cross-protection from Zika virus in a mouse model of infection. Mariah Hassert, Stephen Scroggins, Abigail K. Coleman, Enbal Shacham, James D. Brien, Amelia K. Pinto. bioRxiv 2020.12.23.424273; doi: https://doi.org/10.1101/2020.12.23.424273

SARS-CoV-2 spike protein promotes IL-6 trans-signaling by activation of angiotensin II receptor signaling in epithelial cells. Patra T, Meyer K, Geerling L, Isbell TS, Hoft DF, et al. 2020. PLOS Pathogens 16(12): e1009128. https://doi.org/10.1371/journal.ppat.1009128

Human iPSC-Derived Neuronal Cells From CTBP1-Mutated Patients Reveal Altered Expression of Neurodevelopmental Gene Networks. Vijayalingam S., Ezekiel Uthayashanker R., Xu Fenglian, Subramanian T., Geerling Elizabeth, Hoelscher Brittany, San KayKay, Ganapathy Aravinda, Pemberton Kyle, Tycksen Eric, Pinto Amelia K., Brien James D., Beck David B., Chung Wendy K., Gurnett Christina A., Chinnadurai G. 2020. Frontiers in Neuroscience(14). doi=10.3389/fnins.2020.562292

*Characterization of cells susceptible to SARS-COV-2 and methods for detection of neutralizing antibody by focus forming assay. E. Taylor Stone, Elizabeth Geerling, Tara L. Steffen, Mariah Hassert, Alexandria Dickson, Jacqueline F. Spencer, Karoly Toth, Richard J. DiPaolo, James D. Brien, Amelia K. Pinto. bioRxiv 2020.08.20.259838; doi: https://doi.org/10.1101/2020.08.20.259838

Generation and characterization of an Il2rg knockout Syrian hamster model for XSCID and HAdV-C6 infection in immunocompromised patients. Rong Li, Baoling Ying, Yanan Liu, Jacqueline F. Spencer, Jinxin Miao, Ann E. Tollefson, James D. Brien, Yaohe Wang, William S. M. Wold, Zhongde Wang, Karoly Toth. Disease Models & Mechanisms 2020 13: dmm044602 doi: 10.1242/dmm.044602

Immunogenicity and Efficacy of a Recombinant Human Adenovirus Type 5 Vaccine against Zika Virus. Tara Steffen, Mariah Hassert, Stella G. Hoft, E. Taylor Stone, Jianfeng Zhang, Elizabeth Geerling, Brian T. Grimberg, M. Scot Roberts, Amelia K. Pinto, James D. Brien. Vaccines 2020, 8(2), 170; https://doi.org/10.3390/vaccines8020170

The small molecule AZD6244 inhibits dengue virus replication in vitro and protects against lethal challenge in a mouse model. de Oliveira, L.C., Ribeiro, A.M., Albarnaz, J.D. et al. Arch Virol 165, 671–681 (2020). https://doi.org/10.1007/s00705-020-04524-7

mRNA induced expression of human angiotensin-converting enzyme 2 in mice for the study of the adaptive immune response to severe acute respiratory syndrome coronavirus 2. Hassert M, Geerling E, Stone ET, Steffen TL, Feldman MS, et al. 2020. PLOS Pathogens 16(12): e1009163.https://doi.org/10.1371/journal.ppat.1009163

Diagnostic differentiation of Zika and dengue virus exposure by analyzing T cell receptor sequences from peripheral blood of infected HLA-A2 transgenic mice. Hassert M, Wolf KJ, Rajeh A, Schiebout C, Hoft SG, et al. 2020. PLOS Neglected Tropical Diseases 14(12): e0008896. https://doi.org/10.1371/journal.pntd.0008896

Current Flavivirus Research Important for Vaccine Development. Geerling E, Steffen TL, Brien JD, Pinto AK. Vaccines. 2020; 8(3):477. https://doi.org/10.3390/vaccines8030477

*The receptor binding domain of SARS-CoV-2 spike is the key target of neutralizing antibody in human polyclonal sera. Tara L. Steffen, E. Taylor Stone, Mariah Hassert, Elizabeth Geerling, Brian T. Grimberg, Ana M. Espino, Petraleigh Pantoja, Consuelo Climent, Daniel F. Hoft, Sarah L. George, Carlos A. Sariol, Amelia K. Pinto, James D. Brien. bioRxiv 2020.08.21.261727; doi: https://doi.org/10.1101/2020.08.21.261727

Heterotypic immunity against vaccinia virus in an HLA-B*07:02 transgenic mousepox infection model. Kumar, A., Suryadevara, N.C., Wolf, K.J. et al. Sci Rep 10, 13167 (2020). https://doi.org/10.1038/s41598-020-69897-w

Effective control of early Zika virus replication by Dengue immunity is associated to the length of time between the 2 infections but not mediated by antibodies. Serrano-Collazo C, Pérez-Guzmán EX, Pantoja P, Hassert MA, Rodríguez IV, et al. (2020) PLOS Neglected Tropical Diseases 14(5): e0008285. https://doi.org/10.1371/journal.pntd.0008285

T Cell Responses Induced by Attenuated Flavivirus Vaccination Are Specific and Show Limited Cross-Reactivity with Other Flavivirus Species. Alba Grifoni, Hannah Voic, Sandeep Kumar Dhanda, Conner K. Kidd, James D. Brien, Søren Buus, Anette Stryhn, Anna P. Durbin, Stephen Whitehead, Sean A. Diehl, Aruna D. De Silva, Angel Balmaseda, Eva Harris, Daniela Weiskopf, Alessandro Sette. Journal of Virology May 2020, 94 (10) e00089-20; DOI: 10.1128/JVI.00089-20

Dussupt, V., Sankhala, R.S., Gromowski, G.D. et al. Potent Zika and dengue cross-neutralizing antibodies induced by Zika vaccination in a dengue-experienced donor. Nat Med 26, 228–235 (2020). https://doi.org/10.1038/s41591-019-0746-2

Time elapsed between Zika and dengue virus infections affects antibody and T cell responses. Pérez-Guzmán, E.X., Pantoja, P., Serrano-Collazo, C. et al. Nat Commun 10, 4316 (2019). https://doi.org/10.1038/s41467-019-12295-2

Identification of Protective CD8 T Cell Responses in a Mouse Model of Zika Virus Infection. Hassert Mariah, Harris Madison G., Brien James D., Pinto Amelia K. Frontiers in Immunology 10, 1678. (2019) doi=10.3389/fimmu.2019.01678

*Significant control of Zika infection in macaques depends on the elapsing time after dengue exposure. Crisanta Serrano-Collazo, Erick X. Pérez-Guzmán, Petraleigh Pantoja, Mariah A. Hassert, Idia V. Rodríguez, Luis Giavedoni, Vida Hodara, Laura Parodi, Lorna Cruz, Teresa Arana, Melween I. Martínez, Laura White, James D Brien, Aravinda de Silva, Amelia K. Pinto, Carlos A. Sariol. bioRxiv 625293; doi: https://doi.org/10.1101/625293

Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse. Brien, J. D., Hassert, M., Stone, E. T., Geerling, E., Cruz-Orengo, L., Pinto, A. K. J. Vis. Exp 150, (2019) e59632, doi:10.3791/59632

Mouse Models of Heterologous Flavivirus Immunity: A Role for Cross-Reactive T Cells. Hassert Mariah, Brien James D., Pinto Amelia K. Frontiers in Immunology 10, 1045. (2019) doi=10.3389/fimmu.2019.01045

Time elapsed between Zika and dengue virus infections affects antibody and T cell responses. Pérez-Guzmán, E.X., Pantoja, P., Serrano-Collazo, C. et al. Nat Commun 10, 4316 (2019). https://doi.org/10.1038/s41467-019-12295-2

The Temporal Role of Cytokines in Flavivirus Protection and Pathogenesis. Hassert, M., Brien, J.D. & Pinto, A.K. Curr Clin Micro Rpt 6, 25–33 (2019). https://doi.org/10.1007/s40588-018-0106-x

CD4+T cells mediate protection against Zika associated severe disease in a mouse model of infection. Hassert M, Wolf KJ, Schwetye KE, DiPaolo RJ, Brien JD, et al. (2018) PLOS Pathogens 14(9): e1007237. https://doi.org/10.1371/journal.ppat.1007237

Propagation, Quantification, Detection, and Storage of West Nile Virus. James D. Brien, Helen M. Lazear, Michael S. Diamond. Current Protocols in Microbiology. 31, 1, 15D.3.1-15D.3.18 (2018) https://doi.org/10.1002/9780471729259.mc15d03s31

Preliminary aggregate safety and immunogenicity results from three trials of a purified inactivated Zika virus vaccine candidate: phase 1, randomised, double-blind, placebo-controlled clinical trials. Kayvon Modjarrad, Leyi Lin, Sarah L George, Kathryn E Stephenson, Kenneth H Eckels, Rafael A De La Barrera, Richard G Jarman, Erica Sondergaard, Janice Tennant, Jessica L Ansel, Kristin Mills, Michael Koren, Merlin L Robb, Jill Barrett, Jason Thompson, Alison E Kosel, Peter Dawson, Andrew Hale, C Sabrina Tan, Stephen R Walsh, Keith E Meyer, James Brien, Trevor A Crowell, Azra Blazevic, Karla Mosby, Rafael A Larocca, Peter Abbink, Michael Boyd, Christine A Bricault, Michael S Seaman, Anne Basil, Melissa Walsh, Veronica Tonwe, Daniel F Hoft, Stephen J Thomas, Dan H Barouch, Nelson L Michael. The Lancet 391, 10120, 563-571. (2018) https://doi.org/10.1016/S0140-6736(17)33106-9

Pantoja, P., Pérez-Guzmán, E., Rodríguez, I. et al. Zika virus pathogenesis in rhesus macaques is unaffected by pre-existing immunity to dengue virus. Nat Commun 8, 15674 (2017). https://doi.org/10.1038/ncomms15674

Defining New Therapeutics Using a More Immunocompetent Mouse Model of Antibody-Enhanced Dengue Virus Infection. Amelia K. Pinto, James D. Brien, Chia-Ying Kao Lam, Syd Johnson, Cindy Chiang, John Hiscott, Vanessa V. Sarathy, Alan D. Barrett, Sujan Shresta, Michael S. Diamond. mBio Sep 2015, 6 (5) e01316-15; doi: 10.1128/mBio.01316-15

Human and Murine IFIT1 Proteins Do Not Restrict Infection of Negative-Sense RNA Viruses of the Orthomyxoviridae, Bunyaviridae, and Filoviridae Families. Amelia K. Pinto, Graham D. Williams, Kristy J. Szretter, James P. White, José Luiz Proença-Módena, Gai Liu, Judith Olejnik, James D. Brien, Hideki Ebihara, Elke Mühlberger, Gaya Amarasinghe, Michael S. Diamond, Adrianus C. M. Boon. Journal of Virology Aug 2015, 89 (18) 9465-9476; doi: 10.1128/JVI.00996-15

Isolation and Characterization of Broad and Ultrapotent Human Monoclonal Antibodies with Therapeutic Activity against Chikungunya Virus. Scott A. Smith, Laurie A. Silva, Julie M. Fox, Andrew I. Flyak, Nurgun Kose, Gopal Sapparapu, Solomiia Khomandiak, Alison W. Ashbrook, Kristen M. Kahle, Rachel H. Fong, Sherri Swayne, Benjamin J. Doranz, Charles E. McGee, Mark T. Heise, Pankaj Pal, James D. Brien, S. Kyle Austin, Michael S. Diamond, Terence S. Dermody, James E. Crowe. Cell Host & Microbe. 18, 1, 86-95. (2015) https://doi.org/10.1016/j.chom.2015.06.009

Chikungunya Virus Infection Results in Higher and Persistent Viral Replication in Aged Rhesus Macaques Due to Defects in Anti-Viral Immunity. Ilhem Messaoudi, Jennifer Vomaske, Thomas Totonchy, Craig N. Kreklywich, Kristen Haberthur, Laura Springgay, James D. Brien, Michael S. Diamond, Victor R. DeFilippis, Daniel N. Streblow. (2013) PLOS Neglected Tropical Diseases 7(7): e2343. https://doi.org/10.1371/journal.pntd.0002343

Protection by Immunoglobulin Dual-Affinity Retargeting Antibodies against Dengue Virus. James D. Brien, Soila Sukupolvi-Petty, Katherine L. Williams, Chia-Ying Kao Lam, Michael A. Schmid, Syd Johnson, Eva Harris, Michael S. Diamond. Journal of Virology Jun 2013, 87 (13) 7747-7753; DOI: 10.1128/JVI.00327-13

Functional Analysis of Antibodies against Dengue Virus Type 4 Reveals Strain-Dependent Epitope Exposure That Impacts Neutralization and Protection. Soila Sukupolvi-Petty, James D. Brien, S. Kyle Austin, Bimmi Shrestha, Sherri Swayne, Kristen Kahle, Benjamin J. Doranz, Syd Johnson, Theodore C. Pierson, Daved H. Fremont, Michael S. Diamond. Journal of Virology Jul 2013, 87 (16) 8826-8842; DOI: 10.1128/JVI.01314-13

Development of a Highly Protective Combination Monoclonal Antibody Therapy against Chikungunya Virus. Pal P, Dowd KA, Brien JD, Edeling MA, Gorlatov S, et al. (2013) PLOS Pathogens 9(4): e1003312. https://doi.org/10.1371/journal.ppat.1003312

Cytomegalovirus Infection Impairs Immune Responses and Accentuates T-cell Pool Changes Observed in Mice with Aging. Cicin-Sain L, Brien JD, Uhrlaub JL, Drabig A, Marandu TF, et al. (2012) PLOS Pathogens 8(8): e1002849. https://doi.org/10.1371/journal.ppat.1002849

Shapes Both Innate and CD8+ T Cell Immune Responses against West Nile Virus Infection. Brien JD, Daffis S, Lazear HM, Cho H, Suthar MS, et al. (2011) Interferon Regulatory Factor-1 (IRF-1) PLOS Pathogens 7(9): e1002230. https://doi.org/10.1371/journal.ppat.1002230

The Interferon-Inducible Gene viperin Restricts West Nile Virus Pathogenesis. Kristy J. Szretter, James D. Brien, Larissa B. Thackray, Herbert W. Virgin, Peter Cresswell, Michael S. Diamond. Journal of Virology Oct 2011, 85 (22) 11557-11566; doi: 10.1128/JVI.05519-11

Repeated In Vivo Stimulation of T and B Cell Responses in Old Mice Generates Protective Immunity against Lethal West Nile Virus Encephalitis. Jennifer L. Uhrlaub, James D. Brien, Douglas G. Widman, Peter W. Mason and Janko Nikolich-Žugich J. Immunol April 1, 2011, 186 (7) 3882-3891; doi: https://doi.org/10.4049/jimmunol.1002799

A Temporal Role Of Type I Interferon Signaling in CD8+ T Cell Maturation during Acute West Nile Virus Infection. Pinto AK, Daffis S, Brien JD, Gainey MD, Yokoyama WM, et al. (2011) PLOS Pathogens 7(12): e1002407. https://doi.org/10.1371/journal.ppat.1002407

In-Depth Analysis of the Antibody Response of Individuals Exposed to Primary Dengue Virus Infection. de Alwis R, Beltramello M, Messer WB, Sukupolvi-Petty S, Wahala WMPB, et al. (2011) PLOS Neglected Tropical Diseases 5(6): e1188. https://doi.org/10.1371/journal.pntd.0001188

Genotype-Specific Neutralization and Protection by Antibodies against Dengue Virus Type 3. James D. Brien, S. Kyle Austin, Soila Sukupolvi-Petty, Katie M. O'Brien, Syd Johnson, Daved H. Fremont, Michael S. Diamond. Journal of Virology Sep 2010, 84 (20) 10630-10643; DOI: 10.1128/JVI.01190-10

The Development of Therapeutic Antibodies That Neutralize Homologous and Heterologous Genotypes of Dengue Virus Type 1. Shrestha B, Brien JD, Sukupolvi-Petty S, Austin SK, Edeling MA, et al. (2010) PLOS Pathogens 6(4): e1000823. https://doi.org/10.1371/journal.ppat.1000823

Structure and Function Analysis of Therapeutic Monoclonal Antibodies against Dengue Virus Type 2. Soila Sukupolvi-Petty, S. Kyle Austin, Michael Engle, James D. Brien, Kimberly A. Dowd, Katherine L. Williams, Syd Johnson, Rebeca Rico-Hesse, Eva Harris, Theodore C. Pierson, Daved H. Fremont, Michael S. Diamond. Journal of Virology Aug 2010, 84 (18) 9227-9239; DOI: 10.1128/JVI.01087-10

Inflation and Long-Term Maintenance of CD8 T Cells Responding to a Latent Herpesvirus Depend upon Establishment of Latency and Presence of Viral Antigens. Anna Lang, James D. Brien and Janko Nikolich-Žugich J. Immunol December 15, 2009, 183 (12) 8077-8087; DOI: https://doi.org/10.4049/jimmunol.0801117

Key role of T cell defects in age-related vulnerability to West Nile virus. James D. Brien, Jennifer L. Uhrlaub, Alec Hirsch, Clayton A. Wiley, Janko Nikolich-Žugich. J Exp Med (2009) 206 (12): 2735–2745. doi: https://doi.org/10.1084/jem.20090222

Neutralizing Antibodies Against Dengue Virus Type-1 Map to Domain III of the E Protein and Protect Mice from Lethal Challenge. Shrestha, Bimmi & Brien, James & Sukupolvi-Petty, Soila & O'Brien, Kathryn & Nelson, Steevenson & Pierson, Theodore & Diamond, Michael. The American Journal of Tropical Medicine and Hygiene. (2009). Request Full-Text Here

West Nile Virus Capsid Degradation of Claudin Proteins Disrupts Epithelial Barrier Function. Guruprasad R. Medigeshi, Alec J. Hirsch, James D. Brien, Jennifer L. Uhrlaub, Peter W. Mason, Clayton Wiley, Janko Nikolich-Zugich, Jay A. Nelson. Journal of Virology May 2009, 83 (12) 6125-6134; DOI: 10.1128/JVI.02617-08

West Nile Virus-Specific CD4 T Cells Exhibit Direct Antiviral Cytokine Secretion and Cytotoxicity and Are Sufficient for Antiviral Protection. James D. Brien, Jennifer L. Uhrlaub and Janko Nikolich-Žugich J. Immunol December 15, 2008, 181 (12) 8568-8575; DOI: https://doi.org/10.4049/jimmunol.181.12.8568

Cutting Edge: TLR Ligands Increase TCR Triggering by Slowing Peptide-MHC Class I Decay Rates. Brian D. Rudd, James D. Brien, Miles P. Davenport and Janko Nikolich-Žugich J. Immunol October 15, 2008, 181 (8) 5199-5203; DOI: https://doi.org/10.4049/jimmunol.181.8.5199

Age-Related Dysregulation of CD8+ T Cell Memory Specific for a Persistent Virus Is Independent of Viral Replication. Anna Lang, James D. Brien, Ilhem Messaoudi and Janko Nikolich-Žugich J. Immunol April 1, 2008, 180 (7) 4848-4857; DOI: https://doi.org/10.4049/jimmunol.180.7.4848

Protective capacity and epitope specificity of CD8+ T cells responding to lethal West Nile virus infection. ames D. Brien, Jennifer L. Uhrlaub and Janko Nikolich-Žugich J. European Journal of Immunology June 22, 2007. 37, 7, 1855-1863; doi: https://doi.org/10.1002/eji.200737196

Sultam Thiourea Inhibition of West Nile Virus. Eric Barklis, Amelia Still, Mohammad I. Sabri, Alec J. Hirsch, Janko Nikolich-Zugich, James Brien, Tenzin Choesang Dhenub, Isabel Scholz, Ayna Alfadhli. Antimicrobial Agents and Chemotherapy Jun 2007, 51 (7) 2642-2645; DOI: 10.1128/AAC.00007-07

Activation of Virus-Specific CD8+ T Cells by Lipopolysaccharide-Induced IL-12 and IL-18. Hans-Peter Raué, James D. Brien, Erika Hammarlund and Mark K. Slifka. J Immunol December 1, 2004, 173 (11) 6873-6881; DOI: https://doi.org/10.4049/jimmunol.173.11.6873

James D Brien, Mark Slifka. Protease inhibitors strike a blow to KS progression. Trends in Microbiology. 10, 5, 214. (2002) https://doi.org/10.1016/S0966-842X(02)02358-2

The Role of CD40-CD154 Interaction in Antiviral T Cell-Independent IgG Responses. Eva Szomolanyi-Tsuda, James D. Brien, Jill E. Dorgan, Raymond M. Welsh and Robert L. Garcea. J Immunol June 1, 2000, 164 (11) 5877-5882; DOI: https://doi.org/10.4049/jimmunol.164.11.5877

Mark Slifka, James D Brien. TLR5: guardian of the gut. Trends in Microbiology. 9, 11, 528. (2001) https://doi.org/10.1016/S0966-842X(01)02222-3

Antiviral T-Cell-Independent Type 2 Antibody Responses Induced in Vivo in the Absence of T and NK Cells. Eva Szomolanyi-Tsuda, James D. Brien, Jill E. Dorgan, Robert L. Garcea, Robert T. Woodland, Raymond M. Welsh. Virology. 280, 2, 160-186. (2001) https://doi.org/10.1006/viro.2000.0766.